Oxygen-starved tumor cells have survival advantage that promotes cancer spread 缺氧的腫瘤細胞具有生存優勢，可促進癌症擴散

Editor’s note:  It has been known for long that oxygen is toxic to cancer cells.  Cancer cells are  less likely to survive in an oxygen-rich environment.  However, certain cancer cells in vivo may not be readily attacked by oxygen.  Some agents may help get oxygen into a right position to kill cancer cells.

編者註：我們早就知道氧氣對癌細胞有毒。癌細胞在富含氧氣的環境中存活的可能性較小。但是，體內某些癌細胞可能不容易受到氧氣的攻擊。一些物質可能有助於使氧氣進入適當的位置以殺死癌細胞.

JOHNS HOPKINS MEDICINE

IMAGE: CELLS IN GREEN ARE HYPOXIC CELLS THAT ARE PERMANENTLY MARKED SO THEY CAN BE FOLLOWED THROUGHOUT THE METASTATIC PROCESS.THE RED CELLS HAVE NOT EXPERIENCED HYPOXIA. view more 

CREDIT: YU JUNG SHIN

Using cells from human breast cancers and mouse breast cancer models, researchers at the Johns Hopkins Kimmel Cancer Center say they have significant new evidence that tumor cells exposed to low-oxygen conditions have an advantage when it comes to invading and surviving in the bloodstream.

The experiments mapping the “fate” of the cells in two- and three-dimensional lab-created tissue systems and in live animals specifically showed that cells from a primary cancer exposed to low oxygen levels, or hypoxia, have a four times greater probability of becoming viable circulating tumor cells–and likely spreading to distant tissues–than those under normal oxygen conditions.

The results were described Oct. 24 in the journal Nature Communications.

約翰霍普金斯大學金梅爾癌症中心的研究人員使用人類乳腺癌和小鼠乳腺癌模型的細胞說，他們有重要的新證據表明，暴露於低氧條件下的腫瘤細胞在入侵和生存於血液中具有優勢。

繪製二維和三維實驗室創建的組織系統以及活體動物的“命運”細胞的實驗明確表明，來自暴露於低氧水平或缺氧狀態的原發性癌症的細胞具有四倍的發生機率。 與正常氧氣條件下的循環腫瘤細胞相比，它們成為可行的循環腫瘤細胞，並且可能擴散到遠處的組織。

結果於10月24日發表在《自然通訊》雜誌上。

“Our findings also show that these post-hypoxic cells have six times the probability of forming lung metastases, suggesting that oxygen starvation enhances their metastatic capabilities,” says study leader Daniele Gilkes, Ph.D., assistant professor of oncology and researcher in the breast and ovarian cancer program of the Johns Hopkins Kimmel Cancer Center.

Gilkes and her team also identified a pattern of genetic expression in post-hypoxic cells that appears to help the cells survive oxidative stress when they enter the bloodstream. Some tumor cells retain parts of this genetic signature as a “hypoxic memory” even after they have been reoxygenated, the researchers found.

“Cancer cells tend to become more aggressive as they adapt to low oxygen levels,” says Gilkes, “but we were surprised to find that cells that were exposed to hypoxia in the primary tumor maintained their aggressive features even when they were reoxygenated in the blood.”

In the future, the unique features of the hypoxic cells might be used as biomarkers to identify patients at risk for metastasis, or might be targeted directly by therapies to prevent or limit metastasis, the research team suggested.

Hypoxia occurs in 90% of solid tumors and is known to have an adverse impact on a patient’s prognosis. However, little is known about how tumor cells change in response to low oxygen. Gilkes says most research teams–including her own–grow and experiment with tumor cells using the same oxygen concentrations as normal air.

“This is actually a much higher level of oxygen than what is found in our bodies,” Gilkes says. “For example, the average concentration of oxygen in breast tissue is on the order of 6% to 8%, whereas solid breast tumors have a gradient of oxygen concentrations that reach much less than 1% oxygen in some regions.”

For their new experiments, designed to capture the changes that occur as normal breast cells become malignant, Gilkes and colleagues developed an experimental system that uses oxygen as a switch to make tumor cells “light up” with a fluorescent marker after they are exposed to low oxygen conditions of 0.5% or less, comparable to the levels measured in human tumors.

The study’s first author and member of Gilkes’ lab, Inês Godet, used this marker to follow the fate of these cells as they multiplied and moved around within 2D and 3D tissue “spheres ” and “mini-organs” created in the laboratory, as well as in live mouse models of breast cancer.

Using fluorescence activated cell sorting to capture red or green (oxygen deprived) breast cancer cells, followed by RNA sequencing, the team found that the expression of many gene products, including integrin alpha 10 (ITGA10) and ceruloplasmin (CP) are induced in cells that experienced hypoxia within tumors, but not in cells exposed to hypoxia in the lab. The tumor-based hypoxia pattern was also better at predicting the survival of patients free of distant metastases, they concluded after studying similar genetic expression data from primary tumors from more than 1000 patients with breast cancer.

Among the next questions to answer, say the researchers, are whether post-hypoxic tumor cells at metastatic sites are more resistant to chemotherapy than other cells and whether targeting these post-hypoxic cells will be beneficial for treating patients with metastatic cancers.

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Other Johns Hopkins researchers involved in the study were Yu Jung Shin, Julia Ju, Chae Ye, and Guannan Wang.

The research was supported by the National Institutes of Health under the awards U54-CA210173 R00-CA181352; The V Scholar Foundation, Susan G. Komen Foundation; The Jayne Koskinas Ted Giovanis Foundation for Health and Policy; Cindy Rosencrans Fund for Metastatic Triple-Negative Breast Cancer; The Emerson Collective; and the SKCCC Core Grant award P50CA006973.